Progenesis CoMet analyses LC-MS data to discover and reliably quantify compounds and metabolites that are significantly changing in your samples.

Key benefits

Using Progenesis CoMet, you’ll benefit from:

• streamlined quantification and identification
• visualisation of complex data
• accurate peak picking
• complete data with no missing values
• quantification of compounds
• integrated compound identification search tool
• support for major hardware vendors
• easily integrate results with other bioinformatics data

A comprehensive and streamlined workflow

Progenesis CoMet has been developed to measure the relative abundances of metabolites through the analysis of LC-MS data.

It was developed in response to feedback from metabolomics researchers that their analysis involved jumping between many different applications, some developed in-house, to get a final result. This workflow has been streamlined with straightforward analysis steps for quantification and identification of compound changes within your experiments.

Visualisation of complex data

As soon as you load your LC-MS runs into Progenesis CoMet, the software helps you visualise the raw data at each step in the analysis. Ion intensity maps showing a 2D representation of retention time, m/z and feature intensity as well as mass spectra and chromatogram views provide quality assurance of automatic alignment, peak picking and compound deconvolution on every run.

Accurate peak picking

We have developed a bespoke peak picking algorithm to discern compound ions in complex samples. It can detect and differentiate overlapping compound ions which generates accurate quantification data. You can change the peak picking parameters to ensure optimal detection for each experiment.

Complete data with no missing values

Accurate retention time alignment, so all compound ions are in the same location, and co-detection across all sample runs generates a complete data set. With no missing values, valid multivariate statistical analysis can be applied.

Highly accurate quantification of compounds

Progenesis CoMet provides precise measurements of your compound based on the summed ion intensities. All the compound ions are automatically deconvoluted to provide accurate quantitation of each compound. You can then identify the compounds that have statistically significant abundance changes across groups. The result is a final list of interesting compounds measured within your samples.

Identifying compounds is made simple

MetaScope, our own search tool, is integrated into the software. In a single-click you can search your own data and return compound identifications directly back into the workflow. The results, with a MetaScope compound identification score applied, are automatically associated with the quantified compound ions. Metascope can also search SDF databases and return compound structures alongside identification and quantification data.

Support for all major machine vendors

We support data from Waters, Thermo, Agilent and Bruker instruments in Progenesis CoMet. We also support cross-vendor file formats (e.g. mzXML) so we are platform independent. See our Instrument Types FAQ for more information.

If your instrument or file format isn't supported yet, contact us and we'll see what we can do to help.

Easily integrate results with other bioinformatics data

Progenesis CoMet helps you to put quantification and identification results into a wider biological context. Easy-to-use multivariate statistical tools (e.g. correlation analysis) are integrated into the workflow to analyse protein data. Comprehensive data export options, including raw abundance of every isotope peak for every adduct, also allow you to link analysis results with other ‘omics data or provide input into separate statistical software packages. Results from downstream bioinformatics can be imported back into an analysed experiment, combining all your compound data into a single table to review or share.

Want to learn more?

For more information on Progenesis CoMet and its approach to data analysis, please see the following:

• How Progenesis CoMet works: an overview of how analysis is performed, focussing on the most important technical details that differentiate Progenesis from other software
• Frequently-asked questions: answers to many of the most common questions we're asked

Finally, if you have any questions that are not covered by these pages, please ask us a question. We aim to respond to all enquiries within 1 working day.