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Progenesis LC-MS

A unique approach for label-free LC-MS data analysis
Quantify and identify the significant proteins in your experiment…

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Progenesis LC-MS provides detailed control over the quality of the alignment. The integration of database search results for peptide identification into the quantitative dataset is very convenient including MSMS spectra annotation.

Dr Twan America, Group Leader Proteomics Facility
Wageningen University, Netherlands

Supported data file formats

Bruker Daltonics .d folders

Click to download

You may also need to install the latest version of CompassXtract. This can be downloaded after logging in on the Bruker Daltonics' website.

This is the standard format produced by Bruker Daltonics mass spectrometers. Each sample run is contained within a separate .d folder, which typically contains a file named either analysis.baf or analysis.yep.

To select samples to import, first click the Browse button:

Selecting parent folder

Select the parent folder containing your .d folders and click OK.

The .d folders within the parent will then be shown in the Select samples list box:

Selecting samples

Select the samples you want to import and click Next to move on to the next step.

The next page allows you to include a linear m/z calibration as part of the import process:

m/z calibration

If you have included 2 calibrants of a known mass in your experimental samples, you can use these ions to calibrate the sample m/z values as they are imported. To do this, select Perform m/z calibration and set the expected m/z and charge for the calibrant ions. The other required option are:

  • Intensity threshold: the minimum intensity in counts for a calibrant peak to be used in calibration.
  • m/z window: the minimum m/z range over which the calibration algorithm should search for calibrant peaks.

If you do not want to apply an m/z calibration, clear the Perform m/z calibration checkbox.

Click the Import button to begin the data import.

This importer uses ProteoWizard to access the data in the Bruker Daltonics .d folders, and installs the ProteoWizard libaries as part of this data importer.

See also

More data file formats supported by Progenesis LC-MS.