Waters | Nonlinear Dynamics

Progenesis LC-MS

A unique approach for label-free LC-MS data analysis
Quantify and identify the significant proteins in your experiment…

Supported data file formats

Bruker Daltonics .d folders

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This is the standard format produced by Bruker Daltonics Mass Spectrometers. Each sample run is contained within a separate .d folder which typically contains a file named either analysis.baf or analysis.yep.

To select samples to import, first click Browse

Selecting parent folder

Select the parent folder containing your .d folders and press OK

The .d folders within the parent will then be shown in the Select samples list box.

Selecting samples

Select the samples you wish to import and press Next to move on to the next step.

The next page allows you to include a linear m/z calibration as part of the import process.

m/z calibration

If you have included 2 calibrants of a known mass in your experimental samples you can use these ions to calibrate the sample m/z values as they are imported. To do this check Perform m/z calibration and set the expected m/z and charge for the calibrant ions. The other required option are

  • Intensity threshold The minimum intensity in counts for a calibrant peak to be used in calibration.
  • m/z window The minimum m/z range over which the calibration algorithm should search for calibrant peaks.

If you do not wish to apply an m/z calibration un-check the Perform m/z calibration box.

Press Import to begin the data import.

This importer uses ProteoWizard to access the data in the Bruker Daltonics .d folders, and installs the ProteoWizard libaries as part of this data importer

See also

More data file formats supported by Progenesis LC-MS.